Boar seminal plasma exosomes maintain sperm function by infiltrating into the sperm membrane

Boar seminal plasma exosomes maintain sperm function by infiltrating into the sperm membrane

Seminal plasma ingredients are important for maintenance of sperm viability. This study focuses on the effect of boar seminal plasma exosomes on sperm function during long-term liquid storage. Boar seminal plasma exosomes had typical nano-structure morphology as measured by scanning electron microscopy (SEM) and molecular markers such as AWN, CD9 and CD63 by western blot analysis. The effect on...

Novel Flow Cytometry Analyses of Boar Sperm Viability: Can the Addition of Whole Sperm-Rich Fraction Seminal Plasma to Frozen-Thawed Boar Sperm Affect It?

Boar semen cryopreservation remains a challenge due to the extension of cold shock damage. Thus, many alternatives have emerged to improve the quality of frozen-thawed boar sperm. Although the use of seminal plasma arising from boar sperm-rich fraction (SP-SRF) has shown good efficacy; however, the majority of actual sperm evaluation techniques include a single or dual sperm parameter analysis,...

Emerging Roles of Seminal Plasma in Sperm Function

Seminal plasma is comprised of the extracellular sperm milieu from spermatogenesis in the testes, through to maturation in the epididymis, as well as secretions from the accessory sex glands. Consisting primarily of proteins, this substance has a multitude of functions in mammalian reproduction. Seminal plasma influences sperm physiology, success of semen freezing, migration of spermatozoa thro...

The influence of macro- and microelements in seminal plasma on diluted boar sperm quality

BACKGROUND Growing evidence indicates that macro- and microelements in the seminal plasma of humans and various domestic animals are of great importance due to their roles in sperm metabolism, function, survival and oxidative stress. In the present study, we therefore determined the concentrations of macro- and microelements in fresh boar seminal plasma and their relation to sperm quality param...

mRNA profiling for the identification of sperm and seminal plasma